1. What is Cell Culture Split Ratio?

The split ratio (SR) is a measure of how much a cell culture is divided during subculturing. It represents the ratio of final cell number (Nf) to initial cell number (Ni) and is crucial for maintaining consistent cell culture conditions.

2. How Does the Calculator Work?

The calculator uses the split ratio equation:

Where:

• \( SR \) — Split ratio (dimensionless)
• \( N_f \) — Final cell number (dimensionless)
• \( N_i \) — Initial cell number (dimensionless)

Explanation: The equation calculates how many times the cell population has increased during the culture period before splitting.

3. Importance of Split Ratio Calculation

Details: Proper split ratios are essential for maintaining healthy cell cultures, preventing over-confluence, and ensuring consistent experimental conditions across passages.

4. Using the Calculator

Tips: Enter both final and initial cell numbers as whole numbers. Typical split ratios range from 1:2 to 1:10 depending on cell type and growth characteristics.

5. Frequently Asked Questions (FAQ)

Q1: What is a typical split ratio for common cell lines?
A: Most adherent cell lines are split between 1:3 to 1:6, while fast-growing cells may require higher split ratios (1:8 to 1:10).

Q2: How does split ratio relate to passage number?
A: Split ratio affects how quickly passage numbers increase. Higher split ratios result in slower passage number accumulation.

Q3: Should I adjust split ratios for different culture conditions?
A: Yes, optimal split ratios may vary with media composition, serum concentration, and cell density at splitting.

Q4: How does split ratio affect cell behavior?
A: Too high split ratios may lead to extended lag phases, while too low ratios may cause contact inhibition or differentiation.

Q5: Can I use this for suspension cultures?
A: Yes, though suspension cultures are often described by dilution factor rather than split ratio.